Latest Sterilisation, Culture Media & Pure Culture Techniques Objective Type Questions And Answers

61. Salts of heavy metals used as disinfectants are
a. Thiomersal
b. Phenyl mercury nitrate
c. Mercurochrome
d. All of these
Ans: D

62. Cultures are prepared by penetrating the inoculation loop with suspension into the medium, they are
a. Stock cultures
b. Stabcultures
c. Sub-cultures
d. None of these
Ans: B

63. The principle involved in the streak plate method is
a. Separation
b. Streaking
c. Isolation
d. Dilution
Ans: D

64. Culture media for fungi are
a. Potato dextrose agar (PDA)
b. Sabouraud’s agar
c. Czapekdox agar
d. All of the above
Ans: D

65. Spores of actinomycetes are very sensitive, killed at room temperature of
a. 52oC for 30 min.
b. 65oC for 30 min.
c. 70oC for 30 min.
d. 43oC for 30 min.
Ans: B

66. The term that is used for the bacteria which can withstand pasteurization but does not grow at higher temperatures
a. Thermophiles
b. Extreme thermophiles
c. Thermoduric
d. Facultative thermophiles
Ans: C

67. A common laboratory method of cultivating anaerobic micro-organisms is
a. Gas pack system
b. Brewer jar system
c. Pyrogallic acid over the cotton
d. None of these
Ans: C

68. Alkaliphiles grow at pH value between
a. 1 to 6
b. 6 to 9
c. 1 to 11
d. 7 to 12
Ans: D

69. The micro-organisms grow at high salinity are
a. Osmophiles
b. Halophiles
c. Both a and b
d. None of these
Ans: C

70. Non-lactose fermenting colonies seen on Mac Conkey’s medium are
a. Salmonella typhi
b. Escherichia coli
c. Klebsiella pneumoniae
d. Shigella shigae
Ans: A

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