Common Sterilisation, Culture Media & Pure Culture Techniques Multiple Choice Questions And Answers

21. L-Lysine is produced from
a. Corynebacterium glutamicum
b. Clostridium botulinum
c. Mycobacterium sps
d. Pseudomonas
Ans: A

22. The orderly increase in the quantity of all of the cellular components is known as
a. Reproduction
b. Growth
c. Binary fission
d. None of these
Ans: B

23. Theobacillus thio oxidans grow at pH
a. 7.0
b. 1.0
c. 6.0
d. 9.5
Ans: B

24. Slow freezing requires the conditions
a. 0oC to 15oC for 15 min.
b. – 6 oC to – 10oC for 10 min.
c. – 15oC to 3 to 72 hrs.
d. None of these
Ans: C

25. Discontinuous heating is called
a. Pasteurization
b. Sterilization
c. Fermentation
d. Tindalisation
Ans: D

26. Isolation is
a. Purification of culture
b. Introduction of inoculum
c. Separation of a single colony
d. To grow microorganisms on surfaces
Ans: C

27. The condition required for autoclave
a. 121oC temp.and 15 lbs. pressure for 20 min.
b. 120oC temp.and 20 lbs. pressure for 30 min
c. 150oC temp.for 1 hr.
d. 130oC temp for 2 hr.
Ans: C

28. Lysozyme is effective against
a. Gram negative bacteria
b. Gram positive bacteria
c. Protozoa
d. Helminthes
Ans: B

29. Blood agar medium is
a. Enrichment medium
b. Enriched medium
c. Selective medium
d. Differential medium
Ans: B

30. Infrared radiation is a method of sterilization by
a. Dry heat
b. Moist heat
c. Chemical method
d. Mechanical method
Ans: D

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