Basic Sterilisation, Culture Media & Pure Culture Techniques Multiple Choice Questions And Answers

31. Lyophilization means
a. Sterilization
b. Freeze-drying
c. Burning to ashes
d. Exposure to formation
Ans: B

32. Temperature used for hot air oven is
a. 100oC for 1 hour
b. 120oC for 1 hour
c. 160oC for 1 hour
d. 60oC for 1 hour
Ans: C

33. Phenol co-efficient indicates
a. Efficiency of a disinfectant
b. Dilution of a disinfectant
c. Purity of a disinfectant
d. Quantity of a disinfectant
Ans: A

34. This is an agar plate method and is commonly used for estimation of the number of bacteria in milk.
a. Standard Plate Count (SPC)
b. Spread plate
c. Lawn culture
d. Roll tube method
Ans: A

35. Agar is obtained form
a. Brown algae
b. Red algae
c. Green algae
d. Blue-green algae
Ans: B

36. A gram positive organism which produces swarming on culture medium is
a. Salmonella
b. Clostridium
c. Staphylococci
d. Proteus
Ans: D

37. Enhancement of virulence in bacteria is known as
a. Pathogenicity
b. Attenuation
c. Exaltation
d. Toxigenicity
Ans: C

38. For effective sterilization in an autoclave the temperature obtained is
a. 50oC
b. 100oC
c. 120oC
d. 180oC
Ans: C

39. Spores are killed by
a. 70% alcohol
b. Glutaraldehyde
c. Autoclaving
d. Both b and c
Ans: D

40. Glassware are sterilized by
a. Autoclaving
b. Hot air over
c. Incineration
d. None of these
Ans: B

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